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superscript® iii reverse transcriptase synthesis kit  (Thermo Fisher)


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    Thermo Fisher superscript® iii reverse transcriptase synthesis kit
    Superscript® Iii Reverse Transcriptase Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript%C2%AE+iii+reverse+transcriptase+kit/superscript++iii+reverse+transcriptase+synthesis+kit/pmc12221670-72-18-24
    Average 90 stars, based on 1 article reviews
    superscript® iii reverse transcriptase synthesis kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Tissue response and expression of interleukins (IL)-1ß, IL-6, IL-10 after pulp capping with bioglasses in mice.
    Article Snippet: Reverse transcription of RNA was performed using the SuperScript® III Reverse Transcriptase kit (Life Technologies®, Carlsbad, USA) as per the manufacturer’s protocol.

    Article Title: The infectivity of virus particles from Wolbachia-infected Drosophila.
    Article Snippet: DNAse treatment and cDNA synthesis Two micrograms of RNA sample were used for RQ1 RNase-free DNase treatment (Promega) before cDNA synthesis using SuperScript® III Reverse Transcriptase Kit (Thermo Fisher Scientific Inc) and specific primers (Table 1). cDNA was generated by placing samples in a thermocycler using the following profile: 25 °C for 5 min, 50 °C for 60 min and 70 °C for 15 min.

    Article Title: Biosynthesis of the Central Tricyclic Skeleton of Trichothecene Mycotoxins.
    Article Snippet: Pure LinkTM RNA kit and Superscript III Reverse Transcriptase Kit were purchased from Thermo Fisher Scientific.

    Article Title: Markers for determining tumor hypoxia
    Article Snippet: Ribosomal RNA present was depleted from 5 ug of total RNA using the RiboMinus Eukaryote System (Life technologies). cDNA synthesis was performed using SuperScript® III Reverse Transcriptase kit (Invitrogen).

    Article Title: Stage-specific modulation of multinucleation, fusion, and resorption by the long non-coding RNA DLEU1 and miR-16 in human primary osteoclasts
    Article Snippet: The supernatant containing the RNA was collected and the RNA samples were subsequently used to synthesize cDNA, using random hexamers (Life Technologies) and the SuperScript® III Reverse Transcriptase kit (Life Technologies), as already described [ , ]. qPCR reaction was performed by adding SYBR Green PCR Mastermix (Bio-Rad), nuclease-free water, and the pair of primers corresponding to the transcript of interest (reverse and forward; Supplementary Table ), to the cDNA, as reported [ ].

    Article Title: Treatment of tumors with miRNA targeting CDK4/CDK6
    Article Snippet: One μg of total RNA from each sample was subjected to cDNA synthesis using SuperScript® III Reverse Transcriptase kit (Life Technologies, Grand Island, NY), for detection of CDK4, CDK6, and housekeeping genes.

    Article Title: A ZO-2 scaffolding mechanism regulates the Hippo signalling pathway
    Article Snippet: Five micrograms of total RNA were used for first-strand cDNA synthesis with SuperScript III Reverse Transcriptase Kit (Thermo Fisher Scientific).

    Article Title: IUGA Scientific Meeting 2024 : 49th Annual Meeting -Singapore, June 19-22, 2024.
    Article Snippet: IUGA Scientific Meeting 2024 49th Annual Meeting –Singapore, June 19-22, 2024 © The International Urogynecological Association 2024

    Generated:

    Article Title: Tissue response and expression of interleukins (IL)-1ß, IL-6, IL-10 after pulp capping with bioglasses in mice.
    Article Snippet: Reverse transcription of RNA was performed using the SuperScript® III Reverse Transcriptase kit (Life Technologies®, Carlsbad, USA) as per the manufacturer’s protocol.

    Article Title: The infectivity of virus particles from Wolbachia-infected Drosophila.
    Article Snippet: DNAse treatment and cDNA synthesis Two micrograms of RNA sample were used for RQ1 RNase-free DNase treatment (Promega) before cDNA synthesis using SuperScript® III Reverse Transcriptase Kit (Thermo Fisher Scientific Inc) and specific primers (Table 1). cDNA was generated by placing samples in a thermocycler using the following profile: 25 °C for 5 min, 50 °C for 60 min and 70 °C for 15 min.

    Article Title: Biosynthesis of the Central Tricyclic Skeleton of Trichothecene Mycotoxins.
    Article Snippet: Pure LinkTM RNA kit and Superscript III Reverse Transcriptase Kit were purchased from Thermo Fisher Scientific.

    Article Title: Markers for determining tumor hypoxia
    Article Snippet: Ribosomal RNA present was depleted from 5 ug of total RNA using the RiboMinus Eukaryote System (Life technologies). cDNA synthesis was performed using SuperScript® III Reverse Transcriptase kit (Invitrogen).

    Article Title: Stage-specific modulation of multinucleation, fusion, and resorption by the long non-coding RNA DLEU1 and miR-16 in human primary osteoclasts
    Article Snippet: The supernatant containing the RNA was collected and the RNA samples were subsequently used to synthesize cDNA, using random hexamers (Life Technologies) and the SuperScript® III Reverse Transcriptase kit (Life Technologies), as already described [ , ]. qPCR reaction was performed by adding SYBR Green PCR Mastermix (Bio-Rad), nuclease-free water, and the pair of primers corresponding to the transcript of interest (reverse and forward; Supplementary Table ), to the cDNA, as reported [ ].

    Article Title: Treatment of tumors with miRNA targeting CDK4/CDK6
    Article Snippet: One μg of total RNA from each sample was subjected to cDNA synthesis using SuperScript® III Reverse Transcriptase kit (Life Technologies, Grand Island, NY), for detection of CDK4, CDK6, and housekeeping genes.

    Article Title: A ZO-2 scaffolding mechanism regulates the Hippo signalling pathway
    Article Snippet: Five micrograms of total RNA were used for first-strand cDNA synthesis with SuperScript III Reverse Transcriptase Kit (Thermo Fisher Scientific).

    Article Title: IUGA Scientific Meeting 2024 : 49th Annual Meeting -Singapore, June 19-22, 2024.
    Article Snippet: IUGA Scientific Meeting 2024 49th Annual Meeting –Singapore, June 19-22, 2024 © The International Urogynecological Association 2024

    Real-time Polymerase Chain Reaction:

    Article Title: Tissue response and expression of interleukins (IL)-1ß, IL-6, IL-10 after pulp capping with bioglasses in mice.
    Article Snippet: Reverse transcription of RNA was performed using the SuperScript® III Reverse Transcriptase kit (Life Technologies®, Carlsbad, USA) as per the manufacturer’s protocol.

    Article Title: The infectivity of virus particles from Wolbachia-infected Drosophila.
    Article Snippet: DNAse treatment and cDNA synthesis Two micrograms of RNA sample were used for RQ1 RNase-free DNase treatment (Promega) before cDNA synthesis using SuperScript® III Reverse Transcriptase Kit (Thermo Fisher Scientific Inc) and specific primers (Table 1). cDNA was generated by placing samples in a thermocycler using the following profile: 25 °C for 5 min, 50 °C for 60 min and 70 °C for 15 min.

    Article Title: Biosynthesis of the Central Tricyclic Skeleton of Trichothecene Mycotoxins.
    Article Snippet: Pure LinkTM RNA kit and Superscript III Reverse Transcriptase Kit were purchased from Thermo Fisher Scientific.

    Article Title: Markers for determining tumor hypoxia
    Article Snippet: Ribosomal RNA present was depleted from 5 ug of total RNA using the RiboMinus Eukaryote System (Life technologies). cDNA synthesis was performed using SuperScript® III Reverse Transcriptase kit (Invitrogen).

    Article Title: Stage-specific modulation of multinucleation, fusion, and resorption by the long non-coding RNA DLEU1 and miR-16 in human primary osteoclasts
    Article Snippet: The supernatant containing the RNA was collected and the RNA samples were subsequently used to synthesize cDNA, using random hexamers (Life Technologies) and the SuperScript® III Reverse Transcriptase kit (Life Technologies), as already described [ , ]. qPCR reaction was performed by adding SYBR Green PCR Mastermix (Bio-Rad), nuclease-free water, and the pair of primers corresponding to the transcript of interest (reverse and forward; Supplementary Table ), to the cDNA, as reported [ ].

    Article Title: Treatment of tumors with miRNA targeting CDK4/CDK6
    Article Snippet: One μg of total RNA from each sample was subjected to cDNA synthesis using SuperScript® III Reverse Transcriptase kit (Life Technologies, Grand Island, NY), for detection of CDK4, CDK6, and housekeeping genes.

    Article Title: A ZO-2 scaffolding mechanism regulates the Hippo signalling pathway
    Article Snippet: Five micrograms of total RNA were used for first-strand cDNA synthesis with SuperScript III Reverse Transcriptase Kit (Thermo Fisher Scientific).

    Article Title: IUGA Scientific Meeting 2024 : 49th Annual Meeting -Singapore, June 19-22, 2024.
    Article Snippet: IUGA Scientific Meeting 2024 49th Annual Meeting –Singapore, June 19-22, 2024 © The International Urogynecological Association 2024

    SYBR Green Assay:

    Article Title: Tissue response and expression of interleukins (IL)-1ß, IL-6, IL-10 after pulp capping with bioglasses in mice.
    Article Snippet: Reverse transcription of RNA was performed using the SuperScript® III Reverse Transcriptase kit (Life Technologies®, Carlsbad, USA) as per the manufacturer’s protocol.

    Article Title: The infectivity of virus particles from Wolbachia-infected Drosophila.
    Article Snippet: DNAse treatment and cDNA synthesis Two micrograms of RNA sample were used for RQ1 RNase-free DNase treatment (Promega) before cDNA synthesis using SuperScript® III Reverse Transcriptase Kit (Thermo Fisher Scientific Inc) and specific primers (Table 1). cDNA was generated by placing samples in a thermocycler using the following profile: 25 °C for 5 min, 50 °C for 60 min and 70 °C for 15 min.

    Article Title: Biosynthesis of the Central Tricyclic Skeleton of Trichothecene Mycotoxins.
    Article Snippet: Pure LinkTM RNA kit and Superscript III Reverse Transcriptase Kit were purchased from Thermo Fisher Scientific.

    Article Title: Markers for determining tumor hypoxia
    Article Snippet: Ribosomal RNA present was depleted from 5 ug of total RNA using the RiboMinus Eukaryote System (Life technologies). cDNA synthesis was performed using SuperScript® III Reverse Transcriptase kit (Invitrogen).

    Article Title: Stage-specific modulation of multinucleation, fusion, and resorption by the long non-coding RNA DLEU1 and miR-16 in human primary osteoclasts
    Article Snippet: The supernatant containing the RNA was collected and the RNA samples were subsequently used to synthesize cDNA, using random hexamers (Life Technologies) and the SuperScript® III Reverse Transcriptase kit (Life Technologies), as already described [ , ]. qPCR reaction was performed by adding SYBR Green PCR Mastermix (Bio-Rad), nuclease-free water, and the pair of primers corresponding to the transcript of interest (reverse and forward; Supplementary Table ), to the cDNA, as reported [ ].

    Article Title: Treatment of tumors with miRNA targeting CDK4/CDK6
    Article Snippet: One μg of total RNA from each sample was subjected to cDNA synthesis using SuperScript® III Reverse Transcriptase kit (Life Technologies, Grand Island, NY), for detection of CDK4, CDK6, and housekeeping genes.

    Article Title: A ZO-2 scaffolding mechanism regulates the Hippo signalling pathway
    Article Snippet: Five micrograms of total RNA were used for first-strand cDNA synthesis with SuperScript III Reverse Transcriptase Kit (Thermo Fisher Scientific).

    Article Title: IUGA Scientific Meeting 2024 : 49th Annual Meeting -Singapore, June 19-22, 2024.
    Article Snippet: IUGA Scientific Meeting 2024 49th Annual Meeting –Singapore, June 19-22, 2024 © The International Urogynecological Association 2024

    Polymerase Chain Reaction:

    Article Title: Tissue response and expression of interleukins (IL)-1ß, IL-6, IL-10 after pulp capping with bioglasses in mice.
    Article Snippet: Reverse transcription of RNA was performed using the SuperScript® III Reverse Transcriptase kit (Life Technologies®, Carlsbad, USA) as per the manufacturer’s protocol.

    Article Title: The infectivity of virus particles from Wolbachia-infected Drosophila.
    Article Snippet: DNAse treatment and cDNA synthesis Two micrograms of RNA sample were used for RQ1 RNase-free DNase treatment (Promega) before cDNA synthesis using SuperScript® III Reverse Transcriptase Kit (Thermo Fisher Scientific Inc) and specific primers (Table 1). cDNA was generated by placing samples in a thermocycler using the following profile: 25 °C for 5 min, 50 °C for 60 min and 70 °C for 15 min.

    Article Title: Biosynthesis of the Central Tricyclic Skeleton of Trichothecene Mycotoxins.
    Article Snippet: Pure LinkTM RNA kit and Superscript III Reverse Transcriptase Kit were purchased from Thermo Fisher Scientific.

    Article Title: Markers for determining tumor hypoxia
    Article Snippet: Ribosomal RNA present was depleted from 5 ug of total RNA using the RiboMinus Eukaryote System (Life technologies). cDNA synthesis was performed using SuperScript® III Reverse Transcriptase kit (Invitrogen).

    Article Title: Stage-specific modulation of multinucleation, fusion, and resorption by the long non-coding RNA DLEU1 and miR-16 in human primary osteoclasts
    Article Snippet: The supernatant containing the RNA was collected and the RNA samples were subsequently used to synthesize cDNA, using random hexamers (Life Technologies) and the SuperScript® III Reverse Transcriptase kit (Life Technologies), as already described [ , ]. qPCR reaction was performed by adding SYBR Green PCR Mastermix (Bio-Rad), nuclease-free water, and the pair of primers corresponding to the transcript of interest (reverse and forward; Supplementary Table ), to the cDNA, as reported [ ].

    Article Title: Treatment of tumors with miRNA targeting CDK4/CDK6
    Article Snippet: One μg of total RNA from each sample was subjected to cDNA synthesis using SuperScript® III Reverse Transcriptase kit (Life Technologies, Grand Island, NY), for detection of CDK4, CDK6, and housekeeping genes.

    Article Title: A ZO-2 scaffolding mechanism regulates the Hippo signalling pathway
    Article Snippet: Five micrograms of total RNA were used for first-strand cDNA synthesis with SuperScript III Reverse Transcriptase Kit (Thermo Fisher Scientific).

    Article Title: IUGA Scientific Meeting 2024 : 49th Annual Meeting -Singapore, June 19-22, 2024.
    Article Snippet: IUGA Scientific Meeting 2024 49th Annual Meeting –Singapore, June 19-22, 2024 © The International Urogynecological Association 2024



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